Penurunan Kadar Lignin pada Fermentasi Limbah Kayu Mahoni Menggunakan Phanerochaete chrysosporium

Sri Rulianah, Prayitno Prayitno, Christyfani Sindhuwati, Dessi Ria Ambar Ayu, Khalimatus Sa’diyah

Abstract


Limbah Kayu Mahoni merupakan limbah pertanian jenis kayu keras yang mengandung lignoselulosa (lignin, selulosa, hemiselulosa) yang cukup tinggi. Selulosa sangat berpotensi untuk didegradasi oleh Phanerochaete chrysosporium menjadi glukosa. Tujuan penelitian ini untuk mengetahui pengaruh lama fermentasi dan penambahan serbuk Kayu Mahoni terhadap penurunan kadar lignin pada fermentasi limbah Kayu Mahoni menggunakan kapang Phanerochaete chrysosporium. Penelitian dilakukan dengan cara mengeringkan dan mengecilkan ukuran limbah Kayu Mahoni, kemudian melakukan proses fermentasi limbah Kayu Mahoni dengan Phanerochaete chrysosporium dengan rentang waktu 9, 11, 13, 15, dan 17 hari, dan penambahan limbah serbuk Kayu Mahoni sebanyak 5, 6 dan 7%. Sebelum dan sesudah proses fermentasi dilakukan analisa kadar lignin. Hasil terbaik dari penelitian yaitu penurunan kadar lignin sebesar 85,33 % diperoleh pada lama fermentasi 17 hari dan penambahan serbuk Kayu Mahoni sebanyak 5 %.

Mahogany wood waste is a type of hard wood agricultural waste containing lignocellulose which is quite high. In mahogany wood waste also contains lignin which is quite high, so the level of lignin must be reduced so that the cellulose can be used as glucose. Phanerochaete chrysosporium is a type of mold that is able to degrade lignin, cellulose and hemicellulose simultaneously. The purpose of this study was to determine the effect of fermentation time and the addition of mahogany wood waste to the reduction of lignin content in the fermentation of mahogany wood waste using Phanerochaete chrysosporium molds. The study was conducted by drying and reducing the size of mahogany wood waste, then fermentation of mahogany wood waste with Phanerochaete chrysosporium with a span of 9, 11, 13, 15, and 17 days, and the addition of mahogany wood waste 5, 6 and 7%. Before and after the fermentation process, lignin levels were analyzed. The best results from this study were obtained at 17 days of fermentation and the addition of 5% mahogany wood powder, obtained a decrease in lignin content of 85,33 %.


Keywords


fermentation; lignin; lignocellulose; mahogany; Phanerochaete chrysosporium

Full Text:

PDF

References


A. Meryandini, W. Widosari, B. Maranatha, T. C. Sunarti, N. Rachmania, dan H. Satria, Isolasi Bakteri Selulolitik Dan Karakterisasi Enzimnya, MAKARA Sci. Ser., vol. 13, no. 1, hal. 33–38, 2010.

H. Krisnawati, M. Kallio, dan M. Kannien, Swietenia Macrophylla King Ecology , Silviculture and Productivity. 2011.

S. Rulianah, Z. Irfin, Mufid, Prayitno, Produksi Crude Selulase dari Bahan Baku Ampas Tebu Menggunakan Kapang Phanerochaeta chrysosporium, J.Tek. Kim. Ling., vol. 1, no. 1, hal. 17–27, 2017.

P. H. Suharti, S. Rulianah, Y. Maryanty, B. Irawan, F. Chen, dan M. J. Tsai, The degradation of cellulose in the fermentation of patchouli leaves using Phanerochaete chrysosporium, in Advanced Science Letters, vol. 23, no. 6, hal. 5669–5671, 2017.

G. Sjoberg, Lignin degradation, Swedish University of Agricultural Sciences, 1988.

J. Pérez, J. Muñoz-Dorado, T. De La Rubia, and J. Martínez, Biodegradation and biological treatments of cellulose, hemicellulose and lignin: An overview, Int. Microbiol., vol. 5, no. 2, hal. 53–63, 2002.

M. Tayyab, A. Noman, W. Islam, S. Waheed, Y. Arafat, F. Ali, M. Zaynab, S. Lin, H. Zhang, W. Lin, Bioethanol Production from Lignocellulosic Biomass by Environment-Friendly Pretreatment Methods: A Review, Applied Ecology and Environmental Research, vol. 16, no. 1, hal. 225–249, 2018.

K. T. Steffen, Degradation of Recalcitrant Biopolymers and Polycyclic Aromatic Hydrocarbons by Litter-decomposing Basidiomycetous Fungi, University of Helsinki, 2003.

R. L. Howard, E. Abotsi, E. L. J. van Rensburgand, S. Howard, Lignocellulose Biotechnology: Issues of Bioconversion and Enzyme Production, African Journal of Biotechnology, vol. 2, no. 12, hal. 602–619, 2003.

M. Dashtban, H. Schraft, T. A. Syed, and W. Qin, Fungal biodegradation and enzymatic modification of lignin, Int. J. Biochem. Mol. Biol., vol. 1, no. 1, hal. 36–50, 2010.

Suparjo, degradasi komponen lignoselulosa, 2008.

S. Rulianah, Sindhuwati C., Prayitno, Produksi Crude Selulase dari Limbah Kayu Mahoni Menggunakan Phanerochaete chrysosporium, J. Tek. Kim. Ling, vol. 3, no.1, hal. 39–46, 2019.

G. Janusz, A. Pawlik, J. Sulej, U. Świderska-Burek, A. Jarosz-Wilkolazka, and A. Paszczyński, Lignin degradation: Microorganisms, enzymes involved, genomes analysis and evolution, FEMS Microbiol. Rev., vol. 41, no. 6, hal. 941–962, 2017.

P. J. van Soest, J. B. Robertson, Systems of Analysis for Evaluating Fibrous Feeds, Workshop on Standardization of Analytical Methodology for Feeds, hal. 49–60, 1979.

P. J. van Soest, Plant Fiber and its Role in Herbivore Nutrition, Cornell Vet., vol. 67, no. 3, hal. 307–326, 1977.

H. Krisnawati, E. Varis, M. Kallio, and K. Markku, Ecology , silviculture and productivity, Cent. Int. For. Res., no. November 2014, hal. 1–12, 2011.

A. Imsya, E. B. Laconi, K. G. Wiryawan, and Y. Widyastuti, Biodegradasi Lignoselulosa dengan Phanerochaete chrysosporium terhadap Perubahan Nilai Gizi Pelepah Sawit, J. Peternak. Sriwij., vol. 3, no. 2, hal. 12–19, 2014.

A. Imsya dan R. Palupi, Perubahan Kandungan Lignin , Neutral Detergent Fiber ( NDF ) dan Acid Detergent Fiber ( ADF ) Pelepah Sawit Melalui Proses Biodegumming sebagai Sumber Bahan Pakan Serat Ternak Ruminansia, J. Ilmu Ternak dan Vet., vol. 14, no. 4, hal. 284–287, 2009.

S. Rulianah, H. S. Profiyanty, Y. Maryanty, dan B. Irawan, The effect of fermentation time and addition of CMC to decrease of lignin content in fermentation of patchouli leaves using Phanerochaete chrysosporium, in Advanced Science Letters, 2017, vol. 23, no. 6, hal. 5666–5668.

Fadilah, S. Distantina, S. R. Dwiningsih, dan D. S. Ma’rifah, Pengaruh Penambahan Glukosa Dan Ekstrak Yeast Terhadap Biodelignifikasi Ampas Batang Aren, Ekuilibrium, vol. 8, no. 1, hal. 29-33, 2009.

S. Rulianah dan Hardjono, Pemanfaatan Bagasse Sebagai Crude Selulase Menggunakan Kapang Phanerochaete chrysosporium, in Seminar Rekayasa Kimia dan Proses, 2014.




DOI: http://dx.doi.org/10.33795/jtkl.v4i1.139

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Copyright © 2019 Jurnal Teknik Kimia dan Lingkungan